In research and testing, there are different methods and technologies required for a variety of applications. In the genetic analysis and sequencing, both Sanger sequencing and new-generation sequencing (NSG) is used.
When it comes to Sanger sequencing, the use of an Applied Biosystems sequencer is very common. This equipment is considered to be the standard for most labs and testing facilities, with a long history of providing cutting edge technology along with top features and functions that are designed to improve results and accuracy.
Sanger Sequencing
An Applied Biosystems sequencer that is performing Sanger sequencing will provide rapid results. From test initiation to results in less than a day, the system will be able to provide the information required as well as provide the analysis tools that researchers and technicians are looking for.
The Sanger sequencing uses capillary electrophoresis technology. This is considered the gold standard in sequencing and is used in most facilities around the globe. The Applied Biosystems sequencer manages the workflow in a complete package, limiting the risk of human error or the need to repeat the testing.
The process starts with the sample going through PCR amplification, creating the desired sample size of DNA. Then the cleanup process occurs, removing any fragments. From here, the sequencing occurs, with DNA sequencing dyes marking different ends. Sequencing cleanup then occurs, followed by capillary electrophoresis, which will utilize consumables that are also available through Applied Biosystems.
The ABI Sanger sequencer is a user-friendly piece of equipment that offers both testing capacity as well as software applications for sequencing and fragment analysis. This software is integrated into the system and can be upgraded and patched through any Windows 7 operating system. The company also offers free access to quality check modules for users, allowing quick reporting on the trace quality or to make adjustments to the thresholds as needed.